Isolation and Characterization of Beneficial Bacterium from the Rhizosphere of Suaeda nudiflora in Sundarbans, India

A study was carried out to isolate and characterize salt-tolerant beneficial bacteria from the soil collected rhizosphere of a halophytic plant (Suaeda nudiflora) Gosaba, 24 Parganas (South), West Bengal, India located in Sundarbans delta during January 2018. The physicochemical analysis showed that alkaline nature with high salinity. organic carbon, total nitrogen available phosphorus status were low while potassium content high. Bacterium N8 isolated rhizospheric possessed multiple growth-promoting activities i.e., highest phosphate solubilizing efficiency (8.52 mg P g-1 sugar), nitrogen-fixing capacity (0.7 N phosphatase enzyme activity tolerance salinity at 6% NaCl. morphological this bacterium indicated it gram-positive, rod-shaped present both single chain form. PCR amplification genomic DNA (using 16S rDNA specific primers) clear visible band 1.5Kb. Sequencing amplicon followed by BLAST depicted exhibited similarity (99%) genus Bacillus. NCBI-Gen Bank accession number SUB416213 MH489431. presence members Bacillus Suaeda nudiflora is being reported for first time. This has led significant avenue use as potential bio-inoculum improving nutrient availability coastal region.